Small Group Learning, Introduction to Microbiology: Bacterial Growth and Staining

Description

Students will perform a simple staining technique on bacterial cells to study the morphology. They will also perform a differential staining technique, Gram staining, on bacterial cells and determine if the collected bacteria are Gram-positive, Gram-negative, or both.

Aligned to the Next Generation Science Standards (NGSS)*

Disciplinary Core Ideas: PS1.A; PS1.B; LS1.A

Performance Expectations: MS-LS1-1; MS-LS1-2

Cross Cutting Concepts: Scale, Proportion, and Quantity; Structure and Function

Engineering Practices: Developing and Using Models; Planning and Carrying Out Investigations

Kit Includes:

• 50mL Prepared nutrient agar
• 10mL Methylene Blue
• 10mL Crystal Violet
• 10mL Gram’s Iodine
• 10mL Safranin O
• 30mL Ethanol, 95
• Microscope Slides, pk/72
• Coverslips
• 5 Sterile Petri Dishes
• 5 Sterile Cotton Swabs, pk/2

Regulatory Note: Small Qty Exemption 49CFR173.4

*”Next Generation Science Standards” is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards was involved in the production of, and does not endorse, this product.

 WARNING: This product can expose you to chemicals including Crystal Violet and Methanol/Methyl isobutyl ketone, which are known to the State of California to cause cancer, birth defects and reproductive harm.  For more information go to www.P65Warnings.ca.gov.